Basic and Applied Research Custom Services

Contract Custom Services

VARNISS provides high quality contract custom services in the following areas:

Genetic Engineering, DNA Cloning

Protein Expression, Purification and Refolding

Protein Design, Optimization and Production

Optimization of Lead Therapeutic Proteins

Scientific Technical Consulting

Service (Catalog #)	Input*	Specifications	Output	Timeline	Price from**
PCR Cloning (PCR1)	Insert template and sequence Cloning vector and sequence	Design of PCR Primers Template PCR Vector and Template preparation Ligation, Transformation Sequencing of the clone	Plasmid DNA Glycerol stock Report	1-2 weeks	$887
2-Fragment MegaPCR Cloning (PCR2)	Insert templates and sequence Cloning vector and sequence	Design of PCR Primers Template PCRs, Megaprimer PCR Vector and Template preparation Ligation, Transformation Sequencing of the clone	Plasmid DNA Glycerol stock Report	1-2 weeks	$992
3-Fragment MegaPCR Cloning (PCR3)	Insert templates and sequences Cloning vector and sequence	Design of PCR Primers Template PCRs, Megaprimer PCRs Vector and Template preparation Ligation, Transformation Sequencing of the clone	Plasmid DNA Glycerol stock Report	1-2 weeks	$1,196
Gene Synthesis Cloning (PCR-GS)	Insert DNA Sequence Cloning vector and sequence	Gene Synthesis Design of PCR Primers Template PCR Vector and Template preparation Ligation, Transformation Sequencing of the clone	Plasmid DNA Glycerol stock Report	1-4 weeks	$887 +$0.49/bp
Additional Quality Control by Sequencing (AQCS)		Sequencing of the clone	Report	2-5 days	$189
Transfection Quality Plasmid Isolation: ~500ug, (TQPI500)	Plasmid DNA, E.coli culture or glycerol stock	Growing 150 mL E.coli culture Endotoxin-free plasmid isolation	~500 ug Transfection Quality Plasmid	2 days	$297
Transfection Quality Plasmid Isolation: ~2 mg (TQPI2000)	Plasmid DNA, E.coli culture or glycerol stock	Growing 1 liter E. coli culture Endotoxin-free plasmid isolation	~2 mg Transfection Quality Plasmid	2 days	$498
Protein Expression and Purification: 1 liter of E. coli Culture (PEP1)	Expression Plasmid	Transformation into expression E. coli strain Growing 1 liter of E. coli culture Cell sonication and protein extraction Protein chromatography**** Quality control by SDS PAAG electrophoresis	1-10 mg Protein Report	1 week	$1,396
*Protein Expression, Purification, and Refolding: 1 liter of E. coli* Culture** (PEPR)	Expression Plasmid	Transformation into expression E. coli strain Growing 1 liter E. coli culture Cell sonication and protein extraction Protein chromatography** Quality control by SDS PAAG electrophoresis Protein refolding* Quality control by SDS PAAG electrophoresis	Refolded Protein Report	2 weeks	$1,994
*Growth and Protein Extraction: 1 liter of E. coli* Culture** (EC1)	E. coli culture or glycerol stock	Transformation into expression E. coli strain Growing 1 liter E. coli culture Cell sonication and protein extraction	Protein Extract	4 days	$386
Protein Purification: 1 column chromatography (PP)	Protein Extract	Protein chromatography**** Quality control by SDS PAAG electrophoresis	Purified protein	3 days	$996

*Other input materials are acceptable. **Price is subject to change and depends on the complexity of the projects.

***On-column, arginine micellar, etc. ****Ni, GST, MBP, Affinity, ion exchange, HIC, SEC, etc.

Examples of Purified Proteins

Purified Protein	Reference
p53	http://www.ncbi.nlm.nih.gov/pubmed/18495754
p53 (1-636)	http://www.ncbi.nlm.nih.gov/pubmed/18495754
p63-GST	http://www.ncbi.nlm.nih.gov/pubmed/18495754
p73-GST	http://www.ncbi.nlm.nih.gov/pubmed/18495754
Prothymosin alpha	http://www.ncbi.nlm.nih.gov/pubmed/10903508 http://www.ncbi.nlm.nih.gov/pubmed/9326492
Fis (E.coli)	http://www.ncbi.nlm.nih.gov/pubmed/17617646 http://www.ncbi.nlm.nih.gov/pubmed/14602927
RepA (Bacteriophage P1)	http://www.ncbi.nlm.nih.gov/pubmed/11726699
His6-Z[HER2]-EGFP anti-HER2 Affibody molecule fusion	http://www.ncbi.nlm.nih.gov/pubmed/20052708
His6-Z[HER2]-mCherry anti-HER2 Affibody molecule fusion	http://www.ncbi.nlm.nih.gov/pubmed/20052708
His6-Z[EGF]-EGFP anti-EGF Affibody molecule fusion	http://www.ncbi.nlm.nih.gov/pubmed/20052708
His6-Z[EGF]-mCherry anti-EGF Affibody molecule fusion	http://www.ncbi.nlm.nih.gov/pubmed/20052708
His6-EGF-EGFP	http://www.ncbi.nlm.nih.gov/pubmed/20052708
His6-Z[HER2]-Gly4-Ser-Gly2-Cys	http://www.ncbi.nlm.nih.gov/pubmed/21501640
His6-Z[HER2]-PE38KEDL HER2 Affibody molecule-Pseudomonas Exotoxin A fusion immunotoxin analog	http://www.ncbi.nlm.nih.gov/pubmed/19752752
KillerRed and fusions
T7 RNA Polymerase
NLS-His6-NLS-Venus-TAT
ATPIF1

To request Custom Contract Services, please, contact us and provide a description of the requested service work (desired protocol) for your project. A custom service quote will be sent to you within 48 hours, following the clarification of the requested service work.

Genetic Engineering, DNA Cloning

Suggested Client Input:
Gene or protein name, or sequence
Regulatory regions like promoter, site for activation or repression, terminator
Desired modifications of the sequence
Starting material: DNA, RNA, ORF clone accession number, protein or DNA sequence

VARNISS will conduct:

Design, optimization, and cloning of the protein of interest in bacterial or other expression vectors

Confirm the Clone by DNA sequencing of the insert, and provide 10-20 ug of the purified plasmid and a bacterial stock of the plasmid

Protein Design, Optimization and Production

Suggested Client Input:

Sequence or scheme of protein of interest

List of current and/or potential disadvantages

List of experienced difficulties dealing with protein production, isolation, purification and storage

Expectations regarding protein stability, activity, and purification methodology

VARNISS will conduct:

Design, optimization, cloning, bacterial expression, purification, and refolding (if it is necessary)

Optimization of Lead Therapeutic Proteins

Suggested Client Input:

Protein sequence of the lead candidate

VARNISS will conduct:

Design, optimization, cloning, bacterial expression, purification, and refolding (if it is necessary) of 10 to 100 proteins

Protein Expression, Purification, and Refolding

Suggested Client Input:

Accession number

Bacterial expression vector

Any other expression vector

PCR product containing the desired protein coding part

Organism and protein name

Protein or DNA sequence

Desired modification in the protein-coding or regulatory regions

Recommendations regarding refolding and solubilization of tagged and untagged proteins

VARNISS will conduct:

Design, optimization, cloning, bacterial expression, purification, and refolding (if it is necessary)